FastQCFastQC Report
Wed 13 May 2020
D17_CIPA.forward.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameD17_CIPA.forward.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5391429
Sequences flagged as poor quality0
Sequence length36-298
%GC37

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGGTGACTATAGCAAGGAGGTCACACCTGTTCCCATGCCGAACACAGA905931.6803151817449509No Hit
AAAGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAAGC702891.3037174374363458No Hit
GGGGACGTTCATGGATTCGACAGGGGTCCCCCGAGCTCATTAAGCGTGTC683331.2674376310992874No Hit
AGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAAGGATAT418260.775786901765747No Hit
CAAACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAAC358500.6649443032635689No Hit
TTAAAGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAA226130.41942497990792427No Hit
GTAACACGTGGATAACCTACCTATAAGACTGGGATAACTTCGGGAAACCG160360.29743505849747814No Hit
TCAAACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAA125430.23264704033012398No Hit
AGTAGAGTTAGTTTCCTTGGACTCAGTGCTATGTATTTTTCTTAATTATC115550.21432165757909452No Hit
ACTGAATGACAATATGTCAACGTTAATTCCAAAAACGTAACTATAAGTTA115440.2141176300383442No Hit
TATGAGTACTTTAACTTATGGATTCAACGGTAATGTTACTGGTGATGATA111810.20738472119358337No Hit
AAACAGGGCACCCACCTGTATATAACAGGCCGAATGATCAAGCTATTATA104520.1938632596293116No Hit
ACCAGACTTCGCTACAGTTATTACTATGGATAGAAAAGCATCCAAACAAC100930.18720454261755093No Hit
AGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAAGCTTCGA89660.16630099366976733No Hit
CAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAAGGAT83490.15485690342949893No Hit
GGATCACCTCCTTTCTAAGGATATATTCGGAACATCTTCTTCAGAAGATG80080.14853204966623876No Hit
ACAAGAAACAGCACACATCAATGAATTAACACCTTAGATTCATCATGAGA75040.13918387870822374No Hit
AGTAGAGTTAGTTTCCTTGGACTCAGTGCTATGTATTTTCTTAATTATCA73220.1358081503067183No Hit
GCATTAAGTGGATCATCATATGTGCCGTAGGGTAGCCGAGATTTAGCTAA64470.11957868683794223No Hit
CACTAAAAAATAAGATGAATAATTAATTACTTTCATTGTAAATTTGTTAT62970.11679649310043776No Hit
CAAGCAAAACCGAGTGAATAAAGAGTTTTAAATAAGCTTGAATTCATAAG61660.11436671056968384No Hit
ATATCATTTCAACAATCGGTGACTTAGTAAAATGGATTATCGACACAGTG61510.1140884911959334No Hit
TACTAAACGTAGATGGAACGCTAACCTTCAAAAAGTTAGAATCCTAGTTG61490.11405139527943334No Hit
AACACGTGGATAACCTACCTATAAGACTGGGATAACTTCGGGAAACCGGA57310.10629834873092088No Hit
AGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAAGCTT54890.10180974283441366No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)