FastQCFastQC Report
Wed 13 May 2020
D17C.forward.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameD17C.forward.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7340072
Sequences flagged as poor quality0
Sequence length36-315
%GC36

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAAGC1243521.6941523189418304No Hit
TCTGGTGACTATAGCAAGGAGGTCACACCTGTTCCCATGCCGAACACAGA1074251.4635415020452116No Hit
GGGGACGTTCATGGATTCGACAGGGGTCCCCCGAGCTCATTAAGCGTGTC912771.24354366006219No Hit
CAAACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAAC644850.8785336165639791No Hit
AGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAAGGATAT503380.685797087549005No Hit
TTAAAGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAA388110.5287550312857966No Hit
GTAACACGTGGATAACCTACCTATAAGACTGGGATAACTTCGGGAAACCG277710.3783477873241571No Hit
AGTAGAGTTAGTTTCCTTGGACTCAGTGCTATGTATTTTTCTTAATTATC196790.268103637130535No Hit
TCAAACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAA187740.2557740578021578No Hit
AGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAAGCTTCGA179990.2452155782668072No Hit
TATGAGTACTTTAACTTATGGATTCAACGGTAATGTTACTGGTGATGATA152440.207681886499206No Hit
AGTAGAGTTAGTTTCCTTGGACTCAGTGCTATGTATTTTCTTAATTATCA143450.1954340502381993No Hit
AGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAAGCTT142960.1947664818546739No Hit
ACTGAATGACAATATGTCAACGTTAATTCCAAAAACGTAACTATAAGTTA130970.17843149222514437No Hit
AACTGAATGCTTAGAGCAATCAATTGTTAAAGAAGCTGCGAAAGGTTACT116310.15845893609762957No Hit
GGATCACCTCCTTTCTAAGGATATATTCGGAACATCTTCTTCAGAAGATG113390.1544807734856007No Hit
ATATCATTTCAACAATCGGTGACTTAGTAAAATGGATTATCGACACAGTG108720.14811843807526684No Hit
AACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAACAG108660.14803669500789637No Hit
TAAAGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAAG104300.14209669877897657No Hit
AAGTGAGTAACATTTGCTAGTAGAGTTAGTTTCCTTGGACTCAGTGCTAT102510.1396580306024246No Hit
CACTAAAAAATAAGATGAATAATTAATTACTTTCATTGTAAATTTGTTAT101770.13864986610485566No Hit
AACACGTGGATAACCTACCTATAAGACTGGGATAACTTCGGGAAACCGGA100570.13701500475744652No Hit
GGGGGCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAG97250.13249188836294792No Hit
CAAGCAAAACCGAGTGAATAAAGAGTTTTAAATAAGCTTGAATTCATAAG89990.12260097721112273No Hit
AAACAGGGCACCCACCTGTATATAACAGGCCGAATGATCAAGCTATTATA87720.11950836449560712No Hit
ACCAGACTTCGCTACAGTTATTACTATGGATAGAAAAGCATCCAAACAAC85450.11641575178009153No Hit
ACAAGAAACAGCACACATCAATGAATTAACACCTTAGATTCATCATGAGA82780.11277818528210623No Hit
GAAGGAGTGATTTCAATGGCACAAGATATCATTTCAACAATCGGTGACTT82380.11223323149963652No Hit
CAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAAGGAT81800.11144304851505543No Hit
AAAAATAACTGTAAAACATTCCCTTAATAATAAGTATGGTCGTGAGCCCC81400.11089809473258574No Hit
AAGCAAAACCGAGTGAATAAAGAGTTTTAAATAAGCTTGAATTCATAAGA78220.10656571216195154No Hit
ATTAAAGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAA76420.10411342014083784No Hit
AGGAAACCCTGTAGGTACATTAACTGAATGCTTAGAGCAATCAATTGTTA73960.10076195437864915No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)