FastQCFastQC Report
Wed 13 May 2020
D17B.forward.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameD17B.forward.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8298290
Sequences flagged as poor quality0
Sequence length36-313
%GC36

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAAGC1595931.9232034551696795No Hit
TCTGGTGACTATAGCAAGGAGGTCACACCTGTTCCCATGCCGAACACAGA1086351.3091251330093308No Hit
AGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAAGGATAT912021.0990457070071062No Hit
CAAACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAAC827130.9967475226823839No Hit
GGGGACGTTCATGGATTCGACAGGGGTCCCCCGAGCTCATTAAGCGTGTC726560.8755538791726971No Hit
TTAAAGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAA369660.44546527055574103No Hit
GTAACACGTGGATAACCTACCTATAAGACTGGGATAACTTCGGGAAACCG335590.4044086191251451No Hit
TCAAACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAA288930.34818016723927464No Hit
ACTGAATGACAATATGTCAACGTTAATTCCAAAAACGTAACTATAAGTTA197330.23779597965363947No Hit
TATGAGTACTTTAACTTATGGATTCAACGGTAATGTTACTGGTGATGATA190180.22917974667069962No Hit
AACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAACAG187120.22549223996751136No Hit
CAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAAGGAT177610.21403204756642635No Hit
GGATCACCTCCTTTCTAAGGATATATTCGGAACATCTTCTTCAGAAGATG170280.2051989024244754No Hit
AAGCAAAACCGAGTGAATAAAGAGTTTTAAATAAGCTTGAATTCATAAGA166120.2001858214162195No Hit
AACACGTGGATAACCTACCTATAAGACTGGGATAACTTCGGGAAACCGGA159730.19248543977132637No Hit
AGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAAGCTTCGA150050.18082038588673088No Hit
AGTAGAGTTAGTTTCCTTGGACTCAGTGCTATGTATTTTTCTTAATTATC145370.17518066975244298No Hit
ACAAGAAACAGCACACATCAATGAATTAACACCTTAGATTCATCATGAGA138400.16678134892851418No Hit
CAAGCAAAACCGAGTGAATAAAGAGTTTTAAATAAGCTTGAATTCATAAG119100.14352354521232688No Hit
AGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAAGCTT116770.14071573782068356No Hit
TAAAGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAAG110960.13371429535482612No Hit
AAGTGAGTAACATTTGCTAGTAGAGTTAGTTTCCTTGGACTCAGTGCTAT110950.13370224467932548No Hit
ACTAGATAAGTAAGTAAAATATAGATTTTACCAAGCAAAACCGAGTGAAT105920.12764075490251606No Hit
CACTAAAAAATAAGATGAATAATTAATTACTTTCATTGTAAATTTGTTAT98550.11875940705856267No Hit
GGGGGCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAG96420.11619261317693164No Hit
ACCAGACTTCGCTACAGTTATTACTATGGATAGAAAAGCATCCAAACAAC93880.11313174159977538No Hit
AGTAGAGTTAGTTTCCTTGGACTCAGTGCTATGTATTTTCTTAATTATCA92970.1120351301292194No Hit
ATATCATTTCAACAATCGGTGACTTAGTAAAATGGATTATCGACACAGTG91110.10979370448610498No Hit
GGATCACCTCCTTTCTAAGGATATATTCGGAACATCTTCCTCAGAAGATG90530.10909476530706928No Hit
GCACACATCAATGAATTAACACCTTAGATTCATCATGAGAAAATGCATGG90370.10890195449905944No Hit
AATCAAACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCT89380.10770893762449855No Hit
AACTGAATGCTTAGAGCAATCAATTGTTAAAGAAGCTGCGAAAGGTTACT86810.10461191402084044No Hit
AAACAGGGCACCCACCTGTATATAACAGGCCGAATGATCAAGCTATTATA86430.10415398835181706No Hit
AAATGGAAGTTTGAATCAAAAAACATTAAATGTACCCATGACTTAAATTT86400.10411783632531522No Hit
GCATTAAGTGGATCATCATATGTGCCGTAGGGTAGCCGAGATTTAGCTAA85110.10256329918573584No Hit
GAAGGAGTGATTTCAATGGCACAAGATATCATTTCAACAATCGGTGACTT83760.10093645799315282No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)