FastQCFastQC Report
Wed 13 May 2020
D17A.forward.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameD17A.forward.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7930515
Sequences flagged as poor quality0
Sequence length36-320
%GC35

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAAGC1745902.201496371925405No Hit
CAAACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAAC852121.0744825525202335No Hit
TCTGGTGACTATAGCAAGGAGGTCACACCTGTTCCCATGCCGAACACAGA484990.611549186906525No Hit
TTAAAGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAA447880.5647552523385934No Hit
AGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAAGGATAT434910.5484007028547326No Hit
GGGGACGTTCATGGATTCGACAGGGGTCCCCCGAGCTCATTAAGCGTGTC366420.46203808958182413No Hit
GTAACACGTGGATAACCTACCTATAAGACTGGGATAACTTCGGGAAACCG295160.37218263883240876No Hit
TCAAACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAA281150.35451669910466094No Hit
AGTAGAGTTAGTTTCCTTGGACTCAGTGCTATGTATTTTTCTTAATTATC196660.247978851310413No Hit
TATGAGTACTTTAACTTATGGATTCAACGGTAATGTTACTGGTGATGATA177440.22374335084165403No Hit
AACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAACAG177340.22361725562589566No Hit
AGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAAGCTT156630.19750293644233696No Hit
ACTGAATGACAATATGTCAACGTTAATTCCAAAAACGTAACTATAAGTTA137820.17378442635818733No Hit
AACTGAATGCTTAGAGCAATCAATTGTTAAAGAAGCTGCGAAAGGTTACT136170.1717038552981742No Hit
TAAAGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAAG128430.16194408559847626No Hit
CACTAAAAAATAAGATGAATAATTAATTACTTTCATTGTAAATTTGTTAT128350.16184320942586955No Hit
AAAAATAACTGTAAAACATTCCCTTAATAATAAGTATGGTCGTGAGCCCC122610.15460534404133905No Hit
AAGTGAGTAACATTTGCTAGTAGAGTTAGTTTCCTTGGACTCAGTGCTAT117750.1484771165554822No Hit
ATATCATTTCAACAATCGGTGACTTAGTAAAATGGATTATCGACACAGTG111160.14016744183700555No Hit
AGTAGAGTTAGTTTCCTTGGACTCAGTGCTATGTATTTTCTTAATTATCA102390.12910889141499637No Hit
AACACGTGGATAACCTACCTATAAGACTGGGATAACTTCGGGAAACCGGA101780.1283397105988703No Hit
AAACCCTGTAGGTACATTAACTGAATGCTTAGAGCAATCAATTGTTAAAG101760.12831449155571864No Hit
AAACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCTAACA100730.12701571083340743No Hit
ATTAAAGGAGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAA97870.123409387662718No Hit
GGATCACCTCCTTTCTAAGGATATATTCGGAACATCTTCTTCAGAAGATG96030.12108923569276396No Hit
GAAGGAGTGATTTCAATGGCACAAGATATCATTTCAACAATCGGTGACTT89980.1134604751393825No Hit
CAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTAAGGAT89310.11261563719380141No Hit
ACAAGAAACAGCACACATCAATGAATTAACACCTTAGATTCATCATGAGA86010.10845449507377515No Hit
AATCAAACAATAATTTCGCAGTAGCTGCCTAATCGCACTCTGCATCGCCT82360.1038520196985946No Hit
AGGTACTCATATGGGTAAACAATGTTTCGTAACAGGTCGTAAAGCTTCGA79610.1003844012652394No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)